The Resource Adipose Tissue Protocols, edited by Kaiping Yang, (electronic resource)

Adipose Tissue Protocols, edited by Kaiping Yang, (electronic resource)

Label
Adipose Tissue Protocols
Title
Adipose Tissue Protocols
Statement of responsibility
edited by Kaiping Yang
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly sucessful Methods in Molecular BiologyTM series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers to this burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers
Member of
Dewey number
571.57
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
GP-qlT3A3NQ
Image bit depth
0
Language note
English
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Yang, Kaiping.
Series statement
Methods in Molecular Biology,
Series volume
456
http://library.link/vocab/subjectName
  • Cytology
  • Human physiology
  • Endocrinology
  • Medicine
  • Cell Biology
  • Human Physiology
  • Endocrinology
  • Molecular Medicine
Label
Adipose Tissue Protocols, edited by Kaiping Yang, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders -- Choosing an Adipose Tissue Depot for Sampling -- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass -- Generation of Adipose Tissue-Specific Transgenic Mouse Models -- Angiogenesis in Adipose Tissue -- Adipose Organ Nerves Revealed by Immunohistochemistry# -- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow -- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues -- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue -- Applications of Proteomics to the Study of Adipose Tissue -- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells -- Differentiation of Adipose Stem Cells -- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells -- Explant Cultures of White Adipose Tissue -- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue -- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue -- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells -- Application of RNA Interference Techniques to Adipose Cell Cultures -- RNA Isolation and Real-Time Quantitative RT-PCR -- Study of Adipose Tissue Gene Expression by In Situ Hybridization -- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures -- Determination of Lipolysis in Isolated Primary Adipocytes -- Study of Glucose Uptake in Adipose Cells -- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction
Dimensions
unknown
Extent
1 online resource (XVI, 336 p. 61 illus., 9 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597452458
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-245-8
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000393614
  • (SSID)ssj0000315828
  • (PQKBManifestationID)11278078
  • (PQKBTitleCode)TC0000315828
  • (PQKBWorkID)10257001
  • (PQKB)10486341
  • (DE-He213)978-1-59745-245-8
  • (EXLCZ)991000000000393614
Label
Adipose Tissue Protocols, edited by Kaiping Yang, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders -- Choosing an Adipose Tissue Depot for Sampling -- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass -- Generation of Adipose Tissue-Specific Transgenic Mouse Models -- Angiogenesis in Adipose Tissue -- Adipose Organ Nerves Revealed by Immunohistochemistry# -- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow -- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues -- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue -- Applications of Proteomics to the Study of Adipose Tissue -- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells -- Differentiation of Adipose Stem Cells -- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells -- Explant Cultures of White Adipose Tissue -- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue -- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue -- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells -- Application of RNA Interference Techniques to Adipose Cell Cultures -- RNA Isolation and Real-Time Quantitative RT-PCR -- Study of Adipose Tissue Gene Expression by In Situ Hybridization -- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures -- Determination of Lipolysis in Isolated Primary Adipocytes -- Study of Glucose Uptake in Adipose Cells -- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction
Dimensions
unknown
Extent
1 online resource (XVI, 336 p. 61 illus., 9 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597452458
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-245-8
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000393614
  • (SSID)ssj0000315828
  • (PQKBManifestationID)11278078
  • (PQKBTitleCode)TC0000315828
  • (PQKBWorkID)10257001
  • (PQKB)10486341
  • (DE-He213)978-1-59745-245-8
  • (EXLCZ)991000000000393614

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