The Resource Angiotensin Protocols, edited by Donna H. Wang, (electronic resource)

Angiotensin Protocols, edited by Donna H. Wang, (electronic resource)

Label
Angiotensin Protocols
Title
Angiotensin Protocols
Statement of responsibility
edited by Donna H. Wang
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The renin-angiotensin system has been in the spotlight for more than 100 years because of its vital role in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling. Recent advances in molecular genetic techniques have established that the renin-angiotensin system may well hold the key to the pathogenesis of hypertension, congestive heart failure, chronic renal failure, and stroke. In Angiotensin Protocols, Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing this powerful system. These range from the use of embryonic gene targeting to create mutant strains, to the functional investigation of components of the renin-angiotensin system. Following the successful pattern of the Methods in Molecular BiologyTM series, each method is crafted for experimental success with detailed instructions, perceptive notes highlighting the most critical steps, and helpful tips on pitfalls to avoid. Comprehensive and highly practical, Angiotensin Protocols translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today
Member of
Is Subseries of
Dewey number
612/.015756
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
Q5MKOE_yUzc
Language note
English
LC call number
QP34-38
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Wang, Donna H.
Series statement
Methods in Molecular Medicine,
Series volume
51
http://library.link/vocab/subjectName
  • Human physiology
  • Human Physiology
Label
Angiotensin Protocols, edited by Donna H. Wang, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
to The Renin-Angiotensin System -- Historical Perspective of the Renin –Angiotensin System -- Three Angiotensin Paradigms in One Patient -- Transgenic and Gene Targeting Methods -- Production and Use of Chimeric Mice -- Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase -- Systemic Delivery of aTransgene in Intact Animals by a Retroviral Vector -- Antisense Inhibition of the Renin-Angiotensin System -- Analysis of Transcriptional and Translational Mechanisms -- A nalysis of Transcriptional Control Mechanisms I -- Analysis of Transcriptional Control Mechanisms II Techniques for Characterization of trans-Acting Factors -- Analysis of Cytosolic Proteins that Bind to the 5? Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay -- In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts -- Assays For Determination of Mrna Levels -- Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction -- Analysis of RNA by Northern-Blot Hybridization -- Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs -- In Situ Hybridization -- Assays For Determination of Protein Contents and Enzyme Activity -- Immunohistochemical Detection of Angiotensin II Receptor -- Western Blotting -- Quantification of Angiotensin-Converting Enzyme (ACE) Activity -- Quantification of Angiotensin Peptides -- Radiolabeling of Angiotensin Peptides -- Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors -- Radioligand Binding Assay -- Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues -- Biochemical Studies of The Renin-Angiotensin System -- Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells -- Measurement of Phospholipase D Activation in Vascular Smooth Muscle Cells -- Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor -- Measurement of Nitric Oxide Formation -- Measuring Renin Secretion from Juxtaglomerular Cells -- Functional Studies of The Renin-Angiotensin System -- Measurement of Regional Blood Flow in the Kidney Using Laser-Doppler Flowmetry -- Measurement of Renal Tubular Angiotensin II -- The In Vitro Blood-Perfused Juxtamedullary Nephron Technique -- In Vitro Proximal Tubule Perfusion Preparation -- Isolated Resistance Artery Preparation -- Measurement of Vascular Density -- Functional Studies of the Cerebral Circulation
Dimensions
unknown
Extent
1 online resource (546 p.)
Form of item
online
Isbn
9781592590872
Media category
computer
Media type code
c
Other control number
10.1385/159259087X
Specific material designation
remote
System control number
  • (CKB)1000000000239802
  • (EBL)3036759
  • (SSID)ssj0000103548
  • (PQKBManifestationID)11116981
  • (PQKBTitleCode)TC0000103548
  • (PQKBWorkID)10061157
  • (PQKB)11218633
  • (MiAaPQ)EBC3036759
  • (DE-He213)978-1-59259-087-2
  • (EXLCZ)991000000000239802
Label
Angiotensin Protocols, edited by Donna H. Wang, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
to The Renin-Angiotensin System -- Historical Perspective of the Renin –Angiotensin System -- Three Angiotensin Paradigms in One Patient -- Transgenic and Gene Targeting Methods -- Production and Use of Chimeric Mice -- Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase -- Systemic Delivery of aTransgene in Intact Animals by a Retroviral Vector -- Antisense Inhibition of the Renin-Angiotensin System -- Analysis of Transcriptional and Translational Mechanisms -- A nalysis of Transcriptional Control Mechanisms I -- Analysis of Transcriptional Control Mechanisms II Techniques for Characterization of trans-Acting Factors -- Analysis of Cytosolic Proteins that Bind to the 5? Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay -- In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts -- Assays For Determination of Mrna Levels -- Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction -- Analysis of RNA by Northern-Blot Hybridization -- Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs -- In Situ Hybridization -- Assays For Determination of Protein Contents and Enzyme Activity -- Immunohistochemical Detection of Angiotensin II Receptor -- Western Blotting -- Quantification of Angiotensin-Converting Enzyme (ACE) Activity -- Quantification of Angiotensin Peptides -- Radiolabeling of Angiotensin Peptides -- Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors -- Radioligand Binding Assay -- Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues -- Biochemical Studies of The Renin-Angiotensin System -- Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells -- Measurement of Phospholipase D Activation in Vascular Smooth Muscle Cells -- Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor -- Measurement of Nitric Oxide Formation -- Measuring Renin Secretion from Juxtaglomerular Cells -- Functional Studies of The Renin-Angiotensin System -- Measurement of Regional Blood Flow in the Kidney Using Laser-Doppler Flowmetry -- Measurement of Renal Tubular Angiotensin II -- The In Vitro Blood-Perfused Juxtamedullary Nephron Technique -- In Vitro Proximal Tubule Perfusion Preparation -- Isolated Resistance Artery Preparation -- Measurement of Vascular Density -- Functional Studies of the Cerebral Circulation
Dimensions
unknown
Extent
1 online resource (546 p.)
Form of item
online
Isbn
9781592590872
Media category
computer
Media type code
c
Other control number
10.1385/159259087X
Specific material designation
remote
System control number
  • (CKB)1000000000239802
  • (EBL)3036759
  • (SSID)ssj0000103548
  • (PQKBManifestationID)11116981
  • (PQKBTitleCode)TC0000103548
  • (PQKBWorkID)10061157
  • (PQKB)11218633
  • (MiAaPQ)EBC3036759
  • (DE-He213)978-1-59259-087-2
  • (EXLCZ)991000000000239802

Library Locations

  • Albert D. Cohen Management LibraryBorrow it
    181 Freedman Crescent, Winnipeg, MB, R3T 5V4, CA
    49.807878 -97.129961
  • Architecture/Fine Arts LibraryBorrow it
    84 Curry Place, Winnipeg, MB, CA
    49.807716 -97.136226
  • Archives and Special CollectionsBorrow it
    25 Chancellors Circle (Elizabeth Dafoe Library), Room 330, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Bibliothèque Alfred-Monnin (Université de Saint-Boniface)Borrow it
    200, avenue de la Cathédrale, Local 2110, Winnipeg, MB, R2H 0H7, CA
    49.888861 -97.119735
  • Bill Larson Library (Grace Hospital)Borrow it
    300 Booth Drive, G-227, Winnipeg, MB, R3J 3M7, CA
    49.882400 -97.276436
  • Carolyn Sifton - Helene Fuld Library (St. Boniface General Hospital)Borrow it
    409 Tache Avenue, Winnipeg, MB, R2H 2A6, CA
    49.883388 -97.126050
  • Concordia Hospital LibraryBorrow it
    1095 Concordia Avenue, Winnipeg, MB, R2K 3S8, CA
    49.913252 -97.064683
  • Donald W. Craik Engineering LibraryBorrow it
    75B Chancellors Circle (Engineering Building E3), Room 361, Winnipeg, MB, R3T 2N2, CA
    49.809053 -97.133292
  • E.K. Williams Law LibraryBorrow it
    224 Dysart Road, Winnipeg, MB, R3T 5V4, CA
    49.811829 -97.131017
  • Eckhardt-Gramatté Music LibraryBorrow it
    136 Dafoe Road (Taché Arts Complex), Room 257, Winnipeg, MB, R3T 2N2, CA
    49.807964 -97.132222
  • Elizabeth Dafoe LibraryBorrow it
    25 Chancellors Circle, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Fr. H. Drake Library (St. Paul's College)Borrow it
    70 Dysart Road, Winnipeg, MB, R3T 2M6, CA
    49.810605 -97.138184
  • J.W. Crane Memorial Library (Deer Lodge Centre)Borrow it
    2109 Portage Avenue, Winnipeg, MB, R3J 0L3, CA
    49.878000 -97.235520
  • Libraries Annex (not open to the public; please see web page for details)Borrow it
    25 Chancellors Circle (in the Elizabeth Dafoe Library), Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Neil John Maclean Health Sciences LibraryBorrow it
    727 McDermot Avenue (Brodie Centre), 200 Level, Winnipeg, MB, R3E 3P5, CA
    49.903563 -97.160554
  • Sciences and Technology LibraryBorrow it
    186 Dysart Road, Winnipeg, MB, R3T 2M8, CA
    49.811526 -97.133257
  • Seven Oaks General Hospital LibraryBorrow it
    2300 McPhillips Street, Winnipeg, MB, R2V 3M3, CA
    49.955177 -97.148865
  • Sister St. Odilon Library (Misericordia Health Centre)Borrow it
    99 Cornish Avenue, Winnipeg, MB, R3C 1A2, CA
    49.879592 -97.160425
  • St. John's College LibraryBorrow it
    92 Dysart Road, Winnipeg, MB, R3T 2M5, CA
    49.811242 -97.137156
  • Victoria General Hospital LibraryBorrow it
    2340 Pembina Highway, Winnipeg, MB, R3T 2E8, CA
    49.806755 -97.152739
  • William R Newman Library (Agriculture)Borrow it
    66 Dafoe Road, Winnipeg, MB, R3T 2R3, CA
    49.806936 -97.135525
Processing Feedback ...