The Resource Chromatin Protocols, edited by Srikumar P. Chellappan, (electronic resource)

Chromatin Protocols, edited by Srikumar P. Chellappan, (electronic resource)

Label
Chromatin Protocols
Title
Chromatin Protocols
Statement of responsibility
edited by Srikumar P. Chellappan
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular BiologyTM series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology
Member of
Dewey number
572
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
8tvqI11CL7c
Image bit depth
0
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Chellappan, Srikumar P.
Series statement
Methods in Molecular Biology,
Series volume
523
http://library.link/vocab/subjectName
  • Biochemistry
  • Nucleic acids
  • Gene expression
  • Biochemistry, general
  • Nucleic Acid Chemistry
  • Gene Expression
Label
Chromatin Protocols, edited by Srikumar P. Chellappan, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach -- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins -- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules -- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of DNA Topology in Yeast Chromatin -- Preparation and Analysis of Uniquely Positioned Mononucleosomes -- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy -- Methods to Study Transcription-Coupled Repair in Chromatin -- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs) -- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- In Vitro Replication Assay with Mammalian Cell Extracts -- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction -- Biochemical Analysis of Arginine Methylation in Transcription -- In Vitro Histone Demethylase Assays -- Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation -- Histone Deacetylase Activity Assay -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response
Dimensions
unknown
Edition
2nd ed. 2009.
Extent
1 online resource (XIV, 416 p. 51 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597451901
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-59745-190-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000727077
  • (SSID)ssj0000321831
  • (PQKBManifestationID)11233996
  • (PQKBTitleCode)TC0000321831
  • (PQKBWorkID)10280804
  • (PQKB)10770014
  • (DE-He213)978-1-59745-190-1
  • (EXLCZ)991000000000727077
Label
Chromatin Protocols, edited by Srikumar P. Chellappan, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos -- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach -- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins -- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules -- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues -- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin -- Analysis of DNA Topology in Yeast Chromatin -- Preparation and Analysis of Uniquely Positioned Mononucleosomes -- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy -- Methods to Study Transcription-Coupled Repair in Chromatin -- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs) -- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay -- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes -- In Vitro Replication Assay with Mammalian Cell Extracts -- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction -- Biochemical Analysis of Arginine Methylation in Transcription -- In Vitro Histone Demethylase Assays -- Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation -- Histone Deacetylase Activity Assay -- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination -- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining -- Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo -- ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications -- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays -- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length -- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response
Dimensions
unknown
Edition
2nd ed. 2009.
Extent
1 online resource (XIV, 416 p. 51 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597451901
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-59745-190-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000727077
  • (SSID)ssj0000321831
  • (PQKBManifestationID)11233996
  • (PQKBTitleCode)TC0000321831
  • (PQKBWorkID)10280804
  • (PQKB)10770014
  • (DE-He213)978-1-59745-190-1
  • (EXLCZ)991000000000727077

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