The Resource Cytoskeleton Methods and Protocols, edited by Ray H. Gavin, (electronic resource)

Cytoskeleton Methods and Protocols, edited by Ray H. Gavin, (electronic resource)

Label
Cytoskeleton Methods and Protocols
Title
Cytoskeleton Methods and Protocols
Statement of responsibility
edited by Ray H. Gavin
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls. Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research
Member of
Dewey number
572
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
y17ruG4ffwI
Image bit depth
0
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Gavin, Ray H.
Series statement
Methods in Molecular Biology,
Series volume
586
http://library.link/vocab/subjectName
  • Biochemistry
  • Cytology
  • Cell membranes
  • Microscopy
  • Biochemistry, general
  • Cell Biology
  • Protein Science
  • Membrane Biology
  • Biological Microscopy
Label
Cytoskeleton Methods and Protocols, edited by Ray H. Gavin, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Live-Cell Imaging of the Cytoskeleton -- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis -- Imaging the Cytoskeleton in Live Xenopus laevis Embryos -- Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast -- Dynamics of Actin Filaments and Microtubules -- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization -- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts -- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components -- Quantitative Fluorescence Microscopy Techniques -- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule -- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots -- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells -- Electron Microscopy Protocols for Imaging the Cytoskeleton -- Imaging Cytoskeleton Components by Electron Microscopy -- Purification and Localization of Intraflagellar Transport Particles and Polypeptides -- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies -- Strategies for Imaging Microtubules in Plant Cells -- Analysis of Cell and Organelle Motility -- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity -- Melanosome Motility in Fish Retinal Pigment Epithelial Cells -- Analysis of Properties of Cilia Using Tetrahymena thermophila -- Application of Cell Traction Force Microscopy for Cell Biology Research -- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic and Proteomics Approaches for Cytoskeleton Protein Function -- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions -- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila -- Proteomic Tools for the Analysis of Cytoskeleton Proteins
Dimensions
unknown
Edition
2nd ed. 2010.
Extent
1 online resource (XII, 391 p. 77 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781607613763
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-376-3
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000798333
  • (SSID)ssj0000322429
  • (PQKBManifestationID)11243981
  • (PQKBTitleCode)TC0000322429
  • (PQKBWorkID)10286914
  • (PQKB)10037309
  • (DE-He213)978-1-60761-376-3
  • (EXLCZ)991000000000798333
Label
Cytoskeleton Methods and Protocols, edited by Ray H. Gavin, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Live-Cell Imaging of the Cytoskeleton -- High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis -- Imaging the Cytoskeleton in Live Xenopus laevis Embryos -- Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast -- Dynamics of Actin Filaments and Microtubules -- Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization -- Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts -- Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components -- Quantitative Fluorescence Microscopy Techniques -- A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule -- Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots -- Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells -- Electron Microscopy Protocols for Imaging the Cytoskeleton -- Imaging Cytoskeleton Components by Electron Microscopy -- Purification and Localization of Intraflagellar Transport Particles and Polypeptides -- High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies -- Strategies for Imaging Microtubules in Plant Cells -- Analysis of Cell and Organelle Motility -- A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity -- Melanosome Motility in Fish Retinal Pigment Epithelial Cells -- Analysis of Properties of Cilia Using Tetrahymena thermophila -- Application of Cell Traction Force Microscopy for Cell Biology Research -- 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic and Proteomics Approaches for Cytoskeleton Protein Function -- The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions -- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila -- Proteomic Tools for the Analysis of Cytoskeleton Proteins
Dimensions
unknown
Edition
2nd ed. 2010.
Extent
1 online resource (XII, 391 p. 77 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781607613763
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-376-3
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000798333
  • (SSID)ssj0000322429
  • (PQKBManifestationID)11243981
  • (PQKBTitleCode)TC0000322429
  • (PQKBWorkID)10286914
  • (PQKB)10037309
  • (DE-He213)978-1-60761-376-3
  • (EXLCZ)991000000000798333

Library Locations

  • Albert D. Cohen Management LibraryBorrow it
    181 Freedman Crescent, Winnipeg, MB, R3T 5V4, CA
    49.807878 -97.129961
  • Architecture/Fine Arts LibraryBorrow it
    84 Curry Place, Winnipeg, MB, CA
    49.807716 -97.136226
  • Archives and Special CollectionsBorrow it
    25 Chancellors Circle (Elizabeth Dafoe Library), Room 330, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Bibliothèque Alfred-Monnin (Université de Saint-Boniface)Borrow it
    200, avenue de la Cathédrale, Local 2110, Winnipeg, MB, R2H 0H7, CA
    49.888861 -97.119735
  • Bill Larson Library (Grace Hospital)Borrow it
    300 Booth Drive, G-227, Winnipeg, MB, R3J 3M7, CA
    49.882400 -97.276436
  • Carolyn Sifton - Helene Fuld Library (St. Boniface General Hospital)Borrow it
    409 Tache Avenue, Winnipeg, MB, R2H 2A6, CA
    49.883388 -97.126050
  • Concordia Hospital LibraryBorrow it
    1095 Concordia Avenue, Winnipeg, MB, R2K 3S8, CA
    49.913252 -97.064683
  • Donald W. Craik Engineering LibraryBorrow it
    75B Chancellors Circle (Engineering Building E3), Room 361, Winnipeg, MB, R3T 2N2, CA
    49.809053 -97.133292
  • E.K. Williams Law LibraryBorrow it
    224 Dysart Road, Winnipeg, MB, R3T 5V4, CA
    49.811829 -97.131017
  • Eckhardt-Gramatté Music LibraryBorrow it
    136 Dafoe Road (Taché Arts Complex), Room 257, Winnipeg, MB, R3T 2N2, CA
    49.807964 -97.132222
  • Elizabeth Dafoe LibraryBorrow it
    25 Chancellors Circle, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Fr. H. Drake Library (St. Paul's College)Borrow it
    70 Dysart Road, Winnipeg, MB, R3T 2M6, CA
    49.810605 -97.138184
  • J.W. Crane Memorial Library (Deer Lodge Centre)Borrow it
    2109 Portage Avenue, Winnipeg, MB, R3J 0L3, CA
    49.878000 -97.235520
  • Libraries Annex (not open to the public; please see web page for details)Borrow it
    25 Chancellors Circle (in the Elizabeth Dafoe Library), Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Neil John Maclean Health Sciences LibraryBorrow it
    727 McDermot Avenue (Brodie Centre), 200 Level, Winnipeg, MB, R3E 3P5, CA
    49.903563 -97.160554
  • Sciences and Technology LibraryBorrow it
    186 Dysart Road, Winnipeg, MB, R3T 2M8, CA
    49.811526 -97.133257
  • Seven Oaks General Hospital LibraryBorrow it
    2300 McPhillips Street, Winnipeg, MB, R2V 3M3, CA
    49.955177 -97.148865
  • Sister St. Odilon Library (Misericordia Health Centre)Borrow it
    99 Cornish Avenue, Winnipeg, MB, R3C 1A2, CA
    49.879592 -97.160425
  • St. John's College LibraryBorrow it
    92 Dysart Road, Winnipeg, MB, R3T 2M5, CA
    49.811242 -97.137156
  • Victoria General Hospital LibraryBorrow it
    2340 Pembina Highway, Winnipeg, MB, R3T 2E8, CA
    49.806755 -97.152739
  • William R Newman Library (Agriculture)Borrow it
    66 Dafoe Road, Winnipeg, MB, R3T 2R3, CA
    49.806936 -97.135525
Processing Feedback ...