The Resource FRET and FLIM techniques, edited by T.W.J. Gadella, (electronic resource)

FRET and FLIM techniques, edited by T.W.J. Gadella, (electronic resource)

Label
FRET and FLIM techniques
Title
FRET and FLIM techniques
Statement of responsibility
edited by T.W.J. Gadella
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields
Member of
Is Subseries of
Cataloging source
MiAaPQ
Dewey number
570.282
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QD415.5
LC item number
.L3333 2009
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Gadella, T. W. J.
Series statement
Laboratory techniques in biochemistry and molecular biology
Series volume
v. 33
http://library.link/vocab/subjectName
  • Energy transfer
  • Fluorescence microscopy
Label
FRET and FLIM techniques, edited by T.W.J. Gadella, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Fret and Flim Techniques; Copyright Page; Contents; Contributors; Preface; Chapter 1: Foumlrster resonance energy transfer-FRET what is it, why do it, and how it's done; 1.4. What can we learn from energy transfer?; 1.5. Simple depiction of basic fluorescence; how you determine the FRET efficiency; 1.6. Measuring fluorescence emission of the donor; 1.7. Measuring any de-excitation process in order to determine FRET efficiency; the acronym FRET; 1.8. Measuring photobleaching to determine FRET efficiency; Acknowledgments
  • Chapter 2: Frequency domain FLIM theory, instrumentation, and data analysis2.1. Rates, time constants, and lifetimes; 2.3. Instrumentation: Frequency domain FLIM; 2.4. Systems for measuring lifetimes at multiple frequencies; 2.6. Data acquisition strategies; 2.7. Calibration and measurement validation; 2.9. Calibration by use of reflecting surfaces; 2.10. Calibration by use of fluorophores of known lifetime; 2.11. Comparison of calibration methods; 2.13. First pass analysis-data to modulation depth and phase shift; 2.15. Sine fitting methods for estimating phase and modulation
  • 2.16. Two-component analysis of FLIM data2.17. Application: Semi-quantitative FRET analysis; 2.19. Emerging techniques; Acknowledgments; Chapter 3: Time domain FLIM: Theory, instrumentation, and data analysis; 3.1. Introduction; 3.2. Lifetime detection methods; 3.3. Point scanning time domain FLIM implementations; 3.5. Signal considerations and limitations; 3.6. Data analysis; References; Chapter 4: Multidimensional fluorescence imaging; 4.1. Introduction; 4.2. FLIM and wide-field time-gated imaging; 4.4. Polarization-resolved imaging; Acknowledgments
  • Chapter 5: Visible fluorescent proteins for FRET5.1. Introduction; 5.2. Fluorescent proteins; 5.3. Variants of avGFP; 5.4. Variants of DsRed; 5.5. Variants of eqFP578 and eqFP611; 5.7. Chromoproteins and fluorescent derivatives; 5.8. Optical highlighter fluorescent proteins; 5.9. Choosing the right VFP FRET pair; 5.12. Conclusion; Acknowledgments; References; Chapter 6: Small molecule-based FRET probes; Acknowledgment; Chapter 7: FilterFRET: Quantitative imaging of sensitized emission; 7.1. Introduction; 7.3. Three-channel measurements: FilterFRET; 7.4. Optimizing image acquisition
  • 7.5. Postacquisition improvements and analysis7.6. Discussion; References; Chapter 8: Spectral imaging and its use in the measurement of Foumlrster resonance energy transfer in living cells; 8.1. Introduction; 8.3. What is the spectral signature of FRET?; Chapter 9: Total internal reflection fluorescence lifetime imaging microscopy; 9.1. Introduction: Total internal reflection theory; 9.2. TIRF microscopy history and applications; 9.3. Combining TIRF with FLIM; Acknowledgments; Chapter 10: FRET and FLIM applications in plants; 10.1. Introduction; 10.4. Quantifying FRET-Methods and Pitfalls
  • 10.6. Beyond protein-protein interactions-development and use of FRET-based nanosensors
Dimensions
unknown
Extent
1 online resource (603 p.)
Form of item
online
Isbn
9786612168383
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000747056
  • (EBL)428632
  • (OCoLC)437112440
  • (SSID)ssj0000337894
  • (PQKBManifestationID)11274165
  • (PQKBTitleCode)TC0000337894
  • (PQKBWorkID)10295077
  • (PQKB)10768811
  • (MiAaPQ)EBC428632
  • (EXLCZ)991000000000747056
Label
FRET and FLIM techniques, edited by T.W.J. Gadella, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Fret and Flim Techniques; Copyright Page; Contents; Contributors; Preface; Chapter 1: Foumlrster resonance energy transfer-FRET what is it, why do it, and how it's done; 1.4. What can we learn from energy transfer?; 1.5. Simple depiction of basic fluorescence; how you determine the FRET efficiency; 1.6. Measuring fluorescence emission of the donor; 1.7. Measuring any de-excitation process in order to determine FRET efficiency; the acronym FRET; 1.8. Measuring photobleaching to determine FRET efficiency; Acknowledgments
  • Chapter 2: Frequency domain FLIM theory, instrumentation, and data analysis2.1. Rates, time constants, and lifetimes; 2.3. Instrumentation: Frequency domain FLIM; 2.4. Systems for measuring lifetimes at multiple frequencies; 2.6. Data acquisition strategies; 2.7. Calibration and measurement validation; 2.9. Calibration by use of reflecting surfaces; 2.10. Calibration by use of fluorophores of known lifetime; 2.11. Comparison of calibration methods; 2.13. First pass analysis-data to modulation depth and phase shift; 2.15. Sine fitting methods for estimating phase and modulation
  • 2.16. Two-component analysis of FLIM data2.17. Application: Semi-quantitative FRET analysis; 2.19. Emerging techniques; Acknowledgments; Chapter 3: Time domain FLIM: Theory, instrumentation, and data analysis; 3.1. Introduction; 3.2. Lifetime detection methods; 3.3. Point scanning time domain FLIM implementations; 3.5. Signal considerations and limitations; 3.6. Data analysis; References; Chapter 4: Multidimensional fluorescence imaging; 4.1. Introduction; 4.2. FLIM and wide-field time-gated imaging; 4.4. Polarization-resolved imaging; Acknowledgments
  • Chapter 5: Visible fluorescent proteins for FRET5.1. Introduction; 5.2. Fluorescent proteins; 5.3. Variants of avGFP; 5.4. Variants of DsRed; 5.5. Variants of eqFP578 and eqFP611; 5.7. Chromoproteins and fluorescent derivatives; 5.8. Optical highlighter fluorescent proteins; 5.9. Choosing the right VFP FRET pair; 5.12. Conclusion; Acknowledgments; References; Chapter 6: Small molecule-based FRET probes; Acknowledgment; Chapter 7: FilterFRET: Quantitative imaging of sensitized emission; 7.1. Introduction; 7.3. Three-channel measurements: FilterFRET; 7.4. Optimizing image acquisition
  • 7.5. Postacquisition improvements and analysis7.6. Discussion; References; Chapter 8: Spectral imaging and its use in the measurement of Foumlrster resonance energy transfer in living cells; 8.1. Introduction; 8.3. What is the spectral signature of FRET?; Chapter 9: Total internal reflection fluorescence lifetime imaging microscopy; 9.1. Introduction: Total internal reflection theory; 9.2. TIRF microscopy history and applications; 9.3. Combining TIRF with FLIM; Acknowledgments; Chapter 10: FRET and FLIM applications in plants; 10.1. Introduction; 10.4. Quantifying FRET-Methods and Pitfalls
  • 10.6. Beyond protein-protein interactions-development and use of FRET-based nanosensors
Dimensions
unknown
Extent
1 online resource (603 p.)
Form of item
online
Isbn
9786612168383
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000747056
  • (EBL)428632
  • (OCoLC)437112440
  • (SSID)ssj0000337894
  • (PQKBManifestationID)11274165
  • (PQKBTitleCode)TC0000337894
  • (PQKBWorkID)10295077
  • (PQKB)10768811
  • (MiAaPQ)EBC428632
  • (EXLCZ)991000000000747056

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