The Resource Gene Transfer and Expression in Mammalian Cells, (electronic resource)

Gene Transfer and Expression in Mammalian Cells, (electronic resource)

Label
Gene Transfer and Expression in Mammalian Cells
Title
Gene Transfer and Expression in Mammalian Cells
Title variation
  • New Comprehensive Biochemistry
  • New Comprehensive Biochemistry, Volume 38C
  • Gene Transfer and Expression in Mammalian Cells , Volume 38C
Creator
Contributor
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive
Member of
Is Subseries of
Cataloging source
AU-PeEL
http://library.link/vocab/creatorName
Makrides, S.C
Dewey number
572.86519
Language note
English
LC call number
  • QD415
  • QH
  • QH442
  • QH450
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Makrides, Savvas C
Series statement
New Comprehensive Biochemistry
http://library.link/vocab/subjectName
  • Gene Expression -- physiology
  • Gene expression
  • Genetic engineering
  • Genetic transformation
  • Gene expression
  • Genetic engineering
  • Genetic transformation
  • Genetic Structures
  • Biological Science Disciplines
  • Organisms
  • Genetic Techniques
  • Recombination, Genetic
  • Genetic Processes
  • Investigative Techniques
  • Genetic Phenomena
  • Natural Science Disciplines
  • Analytical, Diagnostic and Therapeutic Techniques and Equipment
  • Phenomena and Processes
  • Disciplines and Occupations
  • Genetic Vectors
  • Gene Expression
  • Methods
  • Physiology
  • Viruses
  • Genetic Engineering
  • Transformation, Genetic
  • Biology
  • Chemistry
  • Health & Biological Sciences
  • Physical Sciences & Mathematics
  • Genetics
  • Biochemistry
Label
Gene Transfer and Expression in Mammalian Cells, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Cover image; Title page; Table of Contents; Preface; Why choose mammalian cells for protein production?; Vectors for gene expression in mammalian cells; 1 Introduction; 2 Transient gene expression; 3 Stable gene expression; 4 Genetic elements of mammalian expression vectors; 5 Selectable markers; 6 Signal peptides and fusion moieties; 7 mRNA and protein stability; 8 Coordinated expression of multiple genes; Virus-based vectors for gene expression in mammalian cells: Herpes simplex virus; 1 Introduction; 2 Herpes Simplex Virus-relevant basic biology; 3 Using HSV-1 to make gene therapy vectors
  • 4 Applications of HSV vectors in the nervous system5 Applications outside the nervous system; 6 Conclusions; Virus-based vectors for gene expression in mammalian cells: Epstein-Barr virus; 1 Introduction; 2 Replication of the EBV genome; 3 EBV-based vectors for gene expression in cell culture; 4 EBV-based vectors for gene therapy; 5 Cell-specific gene expression; 6 A case study for the future; 7 Conclusions; Virus-based vectors for gene expression in mammalian cells: SV40; 1 Introduction; 2 The biology of wild type SV40; 3 Making recombinant SV40 viruses and using them for gene delivery
  • 4 Conclusions and future perspectivesVirus-based vectors for gene expression in mammalian cells: Adeno-associated virus; 1 Biology of adeno-associated virus; 2 Production of recombinant AAV vectors; 3 In vitro and in vivo gene transfer; 4 Immunological aspects of AAV vectors; 5 Development of new AAV vectors; Virus-based vectors for gene expression in mammalian cells: Adenovirus; 1 Introduction; 2 Adenoviral vectors; 3 Gene delivery; 4 Gene expression; 5 Production and analyses of viral particles; 6 Conclusion; Virus-based vectors for gene expression in mammalian cells: Vaccinia virus
  • 1 Introduction2 Vaccinia virus molecular biology; 3 Construction of recombinant poxvirus vectors; 4 Chimeric VV-bacteriophage expression vectors; 5 Improved safety of VV vectors; 6 Non-vaccinia poxvirus vectors; 7 Laboratory and clinical applications; Virus-based vectors for gene expression in mammalian cells: Baculovirus; 1 Introduction; 2 Baculoviruses as insect cell expression vectors; 3 Molecular biology of virus vector construction; 4 Baculoviruses as mammalian cell expression vectors; 5 Characteristics of baculovirus-mediated mammalian gene delivery
  • 6 Applications of the baculovirus mammalian gene delivery vector7 Points to consider; Virus-based vectors for gene expression in mammalian cells: Coronavirus; 1 Introduction; 2 Coronavirus pathogenicity; 3 Molecular biology of coronavirus; 4 Helper-dependent expression systems; 5 Single genome coronavirus vectors; 6 Optimization of transcription levels; 7 Modification of coronavirus tropism and virulence; 8 Expression systems based on arteriviruses; 9 Conclusions; Virus-based vectors for gene expression in mammalian cells: Poliovirus; 1 Introduction; 2 A poliovirus vector-based HIV vaccine
  • 3 Poliovirus vector development and its immunogenic potential
Dimensions
unknown
Extent
1 online resource (1472 p.)
Form of item
electronic
Isbn
9780080930350
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)2500000000003070
  • (EBL)1127741
  • (OCoLC)829461329
  • (SSID)ssj0000809676
  • (PQKBManifestationID)12354093
  • (PQKBTitleCode)TC0000809676
  • (PQKBWorkID)10800673
  • (PQKB)11699305
  • (EXLCZ)992500000000003070
Label
Gene Transfer and Expression in Mammalian Cells, (electronic resource)
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Cover image; Title page; Table of Contents; Preface; Why choose mammalian cells for protein production?; Vectors for gene expression in mammalian cells; 1 Introduction; 2 Transient gene expression; 3 Stable gene expression; 4 Genetic elements of mammalian expression vectors; 5 Selectable markers; 6 Signal peptides and fusion moieties; 7 mRNA and protein stability; 8 Coordinated expression of multiple genes; Virus-based vectors for gene expression in mammalian cells: Herpes simplex virus; 1 Introduction; 2 Herpes Simplex Virus-relevant basic biology; 3 Using HSV-1 to make gene therapy vectors
  • 4 Applications of HSV vectors in the nervous system5 Applications outside the nervous system; 6 Conclusions; Virus-based vectors for gene expression in mammalian cells: Epstein-Barr virus; 1 Introduction; 2 Replication of the EBV genome; 3 EBV-based vectors for gene expression in cell culture; 4 EBV-based vectors for gene therapy; 5 Cell-specific gene expression; 6 A case study for the future; 7 Conclusions; Virus-based vectors for gene expression in mammalian cells: SV40; 1 Introduction; 2 The biology of wild type SV40; 3 Making recombinant SV40 viruses and using them for gene delivery
  • 4 Conclusions and future perspectivesVirus-based vectors for gene expression in mammalian cells: Adeno-associated virus; 1 Biology of adeno-associated virus; 2 Production of recombinant AAV vectors; 3 In vitro and in vivo gene transfer; 4 Immunological aspects of AAV vectors; 5 Development of new AAV vectors; Virus-based vectors for gene expression in mammalian cells: Adenovirus; 1 Introduction; 2 Adenoviral vectors; 3 Gene delivery; 4 Gene expression; 5 Production and analyses of viral particles; 6 Conclusion; Virus-based vectors for gene expression in mammalian cells: Vaccinia virus
  • 1 Introduction2 Vaccinia virus molecular biology; 3 Construction of recombinant poxvirus vectors; 4 Chimeric VV-bacteriophage expression vectors; 5 Improved safety of VV vectors; 6 Non-vaccinia poxvirus vectors; 7 Laboratory and clinical applications; Virus-based vectors for gene expression in mammalian cells: Baculovirus; 1 Introduction; 2 Baculoviruses as insect cell expression vectors; 3 Molecular biology of virus vector construction; 4 Baculoviruses as mammalian cell expression vectors; 5 Characteristics of baculovirus-mediated mammalian gene delivery
  • 6 Applications of the baculovirus mammalian gene delivery vector7 Points to consider; Virus-based vectors for gene expression in mammalian cells: Coronavirus; 1 Introduction; 2 Coronavirus pathogenicity; 3 Molecular biology of coronavirus; 4 Helper-dependent expression systems; 5 Single genome coronavirus vectors; 6 Optimization of transcription levels; 7 Modification of coronavirus tropism and virulence; 8 Expression systems based on arteriviruses; 9 Conclusions; Virus-based vectors for gene expression in mammalian cells: Poliovirus; 1 Introduction; 2 A poliovirus vector-based HIV vaccine
  • 3 Poliovirus vector development and its immunogenic potential
Dimensions
unknown
Extent
1 online resource (1472 p.)
Form of item
electronic
Isbn
9780080930350
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)2500000000003070
  • (EBL)1127741
  • (OCoLC)829461329
  • (SSID)ssj0000809676
  • (PQKBManifestationID)12354093
  • (PQKBTitleCode)TC0000809676
  • (PQKBWorkID)10800673
  • (PQKB)11699305
  • (EXLCZ)992500000000003070

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