The Resource Heterologous Expression of Membrane Proteins : Methods and Protocols, edited by Isabelle Mus-Veteau, (electronic resource)

Heterologous Expression of Membrane Proteins : Methods and Protocols, edited by Isabelle Mus-Veteau, (electronic resource)

Label
Heterologous Expression of Membrane Proteins : Methods and Protocols
Title
Heterologous Expression of Membrane Proteins
Title remainder
Methods and Protocols
Statement of responsibility
edited by Isabelle Mus-Veteau
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field.
Member of
Dewey number
572.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
XbXl4yd4U0g
Image bit depth
0
Language note
English
LC call number
QD431-431.7
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Mus-Veteau, Isabelle.
Series statement
Methods in Molecular Biology,
Series volume
601
http://library.link/vocab/subjectName
  • Biochemistry
  • Cytology
  • Proteomics
  • Cell membranes
  • Protein Science
  • Biochemistry, general
  • Cell Biology
  • Proteomics
  • Membrane Biology
Label
Heterologous Expression of Membrane Proteins : Methods and Protocols, edited by Isabelle Mus-Veteau, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Heterologous Expression of Membrane Proteins for Structural Analysis -- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis -- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli -- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies -- Membrane Protein Expression in Lactococcus lactis -- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae -- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells -- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model -- Membrane Protein Expression in the Eyes of Transgenic Flies -- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors -- Membrane Protein Expression in Cell-Free Systems -- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation -- Membrane Protein Solubilization -- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro -- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (XII, 272 p. 44 illus., 5 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607613442
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-344-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2610000000000024
  • (SSID)ssj0000327909
  • (PQKBManifestationID)11236100
  • (PQKBTitleCode)TC0000327909
  • (PQKBWorkID)10319220
  • (PQKB)11306739
  • (DE-He213)978-1-60761-344-2
  • (EXLCZ)992610000000000024
Label
Heterologous Expression of Membrane Proteins : Methods and Protocols, edited by Isabelle Mus-Veteau, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Heterologous Expression of Membrane Proteins for Structural Analysis -- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis -- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli -- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies -- Membrane Protein Expression in Lactococcus lactis -- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae -- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells -- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model -- Membrane Protein Expression in the Eyes of Transgenic Flies -- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors -- Membrane Protein Expression in Cell-Free Systems -- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation -- Membrane Protein Solubilization -- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro -- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (XII, 272 p. 44 illus., 5 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607613442
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-344-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2610000000000024
  • (SSID)ssj0000327909
  • (PQKBManifestationID)11236100
  • (PQKBTitleCode)TC0000327909
  • (PQKBWorkID)10319220
  • (PQKB)11306739
  • (DE-He213)978-1-60761-344-2
  • (EXLCZ)992610000000000024

Library Locations

  • Albert D. Cohen Management LibraryBorrow it
    181 Freedman Crescent, Winnipeg, MB, R3T 5V4, CA
    49.807878 -97.129961
  • Architecture/Fine Arts LibraryBorrow it
    84 Curry Place, Winnipeg, MB, CA
    49.807716 -97.136226
  • Archives and Special CollectionsBorrow it
    25 Chancellors Circle (Elizabeth Dafoe Library), Room 330, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Bibliothèque Alfred-Monnin (Université de Saint-Boniface)Borrow it
    200, avenue de la Cathédrale, Local 2110, Winnipeg, MB, R2H 0H7, CA
    49.888861 -97.119735
  • Bill Larson Library (Grace Hospital)Borrow it
    300 Booth Drive, G-227, Winnipeg, MB, R3J 3M7, CA
    49.882400 -97.276436
  • Carolyn Sifton - Helene Fuld Library (St. Boniface General Hospital)Borrow it
    409 Tache Avenue, Winnipeg, MB, R2H 2A6, CA
    49.883388 -97.126050
  • Concordia Hospital LibraryBorrow it
    1095 Concordia Avenue, Winnipeg, MB, R2K 3S8, CA
    49.913252 -97.064683
  • Donald W. Craik Engineering LibraryBorrow it
    75B Chancellors Circle (Engineering Building E3), Room 361, Winnipeg, MB, R3T 2N2, CA
    49.809053 -97.133292
  • E.K. Williams Law LibraryBorrow it
    224 Dysart Road, Winnipeg, MB, R3T 5V4, CA
    49.811829 -97.131017
  • Eckhardt-Gramatté Music LibraryBorrow it
    136 Dafoe Road (Taché Arts Complex), Room 257, Winnipeg, MB, R3T 2N2, CA
    49.807964 -97.132222
  • Elizabeth Dafoe LibraryBorrow it
    25 Chancellors Circle, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Fr. H. Drake Library (St. Paul's College)Borrow it
    70 Dysart Road, Winnipeg, MB, R3T 2M6, CA
    49.810605 -97.138184
  • J.W. Crane Memorial Library (Deer Lodge Centre)Borrow it
    2109 Portage Avenue, Winnipeg, MB, R3J 0L3, CA
    49.878000 -97.235520
  • Libraries Annex (not open to the public; please see web page for details)Borrow it
    25 Chancellors Circle (in the Elizabeth Dafoe Library), Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Neil John Maclean Health Sciences LibraryBorrow it
    727 McDermot Avenue (Brodie Centre), 200 Level, Winnipeg, MB, R3E 3P5, CA
    49.903563 -97.160554
  • Sciences and Technology LibraryBorrow it
    186 Dysart Road, Winnipeg, MB, R3T 2M8, CA
    49.811526 -97.133257
  • Seven Oaks General Hospital LibraryBorrow it
    2300 McPhillips Street, Winnipeg, MB, R2V 3M3, CA
    49.955177 -97.148865
  • Sister St. Odilon Library (Misericordia Health Centre)Borrow it
    99 Cornish Avenue, Winnipeg, MB, R3C 1A2, CA
    49.879592 -97.160425
  • St. John's College LibraryBorrow it
    92 Dysart Road, Winnipeg, MB, R3T 2M5, CA
    49.811242 -97.137156
  • Victoria General Hospital LibraryBorrow it
    2340 Pembina Highway, Winnipeg, MB, R3T 2E8, CA
    49.806755 -97.152739
  • William R Newman Library (Agriculture)Borrow it
    66 Dafoe Road, Winnipeg, MB, R3T 2R3, CA
    49.806936 -97.135525
Processing Feedback ...