The Resource Human Embryonic Stem Cell Protocols, edited by Kursad Turksen, (electronic resource)

Human Embryonic Stem Cell Protocols, edited by Kursad Turksen, (electronic resource)

Label
Human Embryonic Stem Cell Protocols
Title
Human Embryonic Stem Cell Protocols
Statement of responsibility
edited by Kursad Turksen
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today
Member of
Dewey number
571.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
DMyHMxpXLKA
Image bit depth
0
Language note
English
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Turksen, Kursad.
Series statement
Methods in Molecular Biology,
Series volume
584
http://library.link/vocab/subjectName
  • Cytology
  • Stem cells
  • Cell culture
  • Cell Biology
  • Stem Cells
  • Cell Culture
Label
Human Embryonic Stem Cell Protocols, edited by Kursad Turksen, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC) -- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos -- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts -- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol -- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency -- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells -- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications -- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation -- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Study of Gap Junctions in Human Embryonic Stem Cells -- hESC Engineering by Integrase-Mediated Chromosomal Targeting -- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts -- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells -- Embryonic Stem Cells as a Model for Studying Melanocyte Development -- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells -- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds -- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells -- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media -- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions
Dimensions
unknown
Edition
2nd ed. 2010.
Extent
1 online resource (X, 444 p. 91 illus., 54 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607613695
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-369-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000804873
  • (SSID)ssj0000327911
  • (PQKBManifestationID)11260904
  • (PQKBTitleCode)TC0000327911
  • (PQKBWorkID)10303475
  • (PQKB)10185910
  • (DE-He213)978-1-60761-369-5
  • (EXLCZ)991000000000804873
Label
Human Embryonic Stem Cell Protocols, edited by Kursad Turksen, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC) -- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos -- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts -- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol -- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency -- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells -- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications -- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation -- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Study of Gap Junctions in Human Embryonic Stem Cells -- hESC Engineering by Integrase-Mediated Chromosomal Targeting -- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts -- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells -- Embryonic Stem Cells as a Model for Studying Melanocyte Development -- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells -- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds -- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells -- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media -- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions
Dimensions
unknown
Edition
2nd ed. 2010.
Extent
1 online resource (X, 444 p. 91 illus., 54 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607613695
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-369-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000804873
  • (SSID)ssj0000327911
  • (PQKBManifestationID)11260904
  • (PQKBTitleCode)TC0000327911
  • (PQKBWorkID)10303475
  • (PQKB)10185910
  • (DE-He213)978-1-60761-369-5
  • (EXLCZ)991000000000804873

Library Locations

  • Albert D. Cohen Management LibraryBorrow it
    181 Freedman Crescent, Winnipeg, MB, R3T 5V4, CA
    49.807878 -97.129961
  • Architecture/Fine Arts LibraryBorrow it
    84 Curry Place, Winnipeg, MB, CA
    49.807716 -97.136226
  • Archives and Special CollectionsBorrow it
    25 Chancellors Circle (Elizabeth Dafoe Library), Room 330, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Bibliothèque Alfred-Monnin (Université de Saint-Boniface)Borrow it
    200, avenue de la Cathédrale, Local 2110, Winnipeg, MB, R2H 0H7, CA
    49.888861 -97.119735
  • Bill Larson Library (Grace Hospital)Borrow it
    300 Booth Drive, G-227, Winnipeg, MB, R3J 3M7, CA
    49.882400 -97.276436
  • Carolyn Sifton - Helene Fuld Library (St. Boniface General Hospital)Borrow it
    409 Tache Avenue, Winnipeg, MB, R2H 2A6, CA
    49.883388 -97.126050
  • Concordia Hospital LibraryBorrow it
    1095 Concordia Avenue, Winnipeg, MB, R2K 3S8, CA
    49.913252 -97.064683
  • Donald W. Craik Engineering LibraryBorrow it
    75B Chancellors Circle (Engineering Building E3), Room 361, Winnipeg, MB, R3T 2N2, CA
    49.809053 -97.133292
  • E.K. Williams Law LibraryBorrow it
    224 Dysart Road, Winnipeg, MB, R3T 5V4, CA
    49.811829 -97.131017
  • Eckhardt-Gramatté Music LibraryBorrow it
    136 Dafoe Road (Taché Arts Complex), Room 257, Winnipeg, MB, R3T 2N2, CA
    49.807964 -97.132222
  • Elizabeth Dafoe LibraryBorrow it
    25 Chancellors Circle, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Fr. H. Drake Library (St. Paul's College)Borrow it
    70 Dysart Road, Winnipeg, MB, R3T 2M6, CA
    49.810605 -97.138184
  • J.W. Crane Memorial Library (Deer Lodge Centre)Borrow it
    2109 Portage Avenue, Winnipeg, MB, R3J 0L3, CA
    49.878000 -97.235520
  • Libraries Annex (not open to the public; please see web page for details)Borrow it
    25 Chancellors Circle (in the Elizabeth Dafoe Library), Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Neil John Maclean Health Sciences LibraryBorrow it
    727 McDermot Avenue (Brodie Centre), 200 Level, Winnipeg, MB, R3E 3P5, CA
    49.903563 -97.160554
  • Sciences and Technology LibraryBorrow it
    186 Dysart Road, Winnipeg, MB, R3T 2M8, CA
    49.811526 -97.133257
  • Seven Oaks General Hospital LibraryBorrow it
    2300 McPhillips Street, Winnipeg, MB, R2V 3M3, CA
    49.955177 -97.148865
  • Sister St. Odilon Library (Misericordia Health Centre)Borrow it
    99 Cornish Avenue, Winnipeg, MB, R3C 1A2, CA
    49.879592 -97.160425
  • St. John's College LibraryBorrow it
    92 Dysart Road, Winnipeg, MB, R3T 2M5, CA
    49.811242 -97.137156
  • Victoria General Hospital LibraryBorrow it
    2340 Pembina Highway, Winnipeg, MB, R3T 2E8, CA
    49.806755 -97.152739
  • William R Newman Library (Agriculture)Borrow it
    66 Dafoe Road, Winnipeg, MB, R3T 2R3, CA
    49.806936 -97.135525
Processing Feedback ...