The Resource Inositol Phosphates and Lipids : Methods and Protocols, edited by Christopher J. Barker, (electronic resource)

Inositol Phosphates and Lipids : Methods and Protocols, edited by Christopher J. Barker, (electronic resource)

Label
Inositol Phosphates and Lipids : Methods and Protocols
Title
Inositol Phosphates and Lipids
Title remainder
Methods and Protocols
Statement of responsibility
edited by Christopher J. Barker
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide
Member of
Dewey number
572/.57
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
nkO4yyL4rk0
Image bit depth
0
Language note
English
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Barker, Christopher J.
Series statement
Methods in Molecular Biology,
Series volume
645
http://library.link/vocab/subjectName
  • Cytology
  • Cell physiology
  • Cell Biology
  • Cell Physiology
Label
Inositol Phosphates and Lipids : Methods and Protocols, edited by Christopher J. Barker, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells -- HPLC Separation of Inositol Polyphosphates -- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells -- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate -- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) -- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7) -- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry -- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio -- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference -- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus -- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides -- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC -- Phosphoinositide Analysis by Liquid Chromatography–Mass Spectrometry -- Imaging Phosphoinositide Dynamics in Living Cells
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (X, 230 p. 54 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781603271752
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60327-175-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000065675
  • (SSID)ssj0000449698
  • (PQKBManifestationID)11314356
  • (PQKBTitleCode)TC0000449698
  • (PQKBWorkID)10433984
  • (PQKB)11079107
  • (DE-He213)978-1-60327-175-2
  • (EXLCZ)992670000000065675
Label
Inositol Phosphates and Lipids : Methods and Protocols, edited by Christopher J. Barker, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells -- HPLC Separation of Inositol Polyphosphates -- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells -- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate -- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) -- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7) -- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry -- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio -- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference -- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus -- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides -- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC -- Phosphoinositide Analysis by Liquid Chromatography–Mass Spectrometry -- Imaging Phosphoinositide Dynamics in Living Cells
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (X, 230 p. 54 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781603271752
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60327-175-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000065675
  • (SSID)ssj0000449698
  • (PQKBManifestationID)11314356
  • (PQKBTitleCode)TC0000449698
  • (PQKBWorkID)10433984
  • (PQKB)11079107
  • (DE-He213)978-1-60327-175-2
  • (EXLCZ)992670000000065675

Library Locations

  • Albert D. Cohen Management LibraryBorrow it
    181 Freedman Crescent, Winnipeg, MB, R3T 5V4, CA
    49.807878 -97.129961
  • Architecture/Fine Arts LibraryBorrow it
    84 Curry Place, Winnipeg, MB, CA
    49.807716 -97.136226
  • Archives and Special CollectionsBorrow it
    25 Chancellors Circle (Elizabeth Dafoe Library), Room 330, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Bibliothèque Alfred-Monnin (Université de Saint-Boniface)Borrow it
    200, avenue de la Cathédrale, Local 2110, Winnipeg, MB, R2H 0H7, CA
    49.888861 -97.119735
  • Bill Larson Library (Grace Hospital)Borrow it
    300 Booth Drive, G-227, Winnipeg, MB, R3J 3M7, CA
    49.882400 -97.276436
  • Carolyn Sifton - Helene Fuld Library (St. Boniface General Hospital)Borrow it
    409 Tache Avenue, Winnipeg, MB, R2H 2A6, CA
    49.883388 -97.126050
  • Concordia Hospital LibraryBorrow it
    1095 Concordia Avenue, Winnipeg, MB, R2K 3S8, CA
    49.913252 -97.064683
  • Donald W. Craik Engineering LibraryBorrow it
    75B Chancellors Circle (Engineering Building E3), Room 361, Winnipeg, MB, R3T 2N2, CA
    49.809053 -97.133292
  • E.K. Williams Law LibraryBorrow it
    224 Dysart Road, Winnipeg, MB, R3T 5V4, CA
    49.811829 -97.131017
  • Eckhardt-Gramatté Music LibraryBorrow it
    136 Dafoe Road (Taché Arts Complex), Room 257, Winnipeg, MB, R3T 2N2, CA
    49.807964 -97.132222
  • Elizabeth Dafoe LibraryBorrow it
    25 Chancellors Circle, Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Fr. H. Drake Library (St. Paul's College)Borrow it
    70 Dysart Road, Winnipeg, MB, R3T 2M6, CA
    49.810605 -97.138184
  • J.W. Crane Memorial Library (Deer Lodge Centre)Borrow it
    2109 Portage Avenue, Winnipeg, MB, R3J 0L3, CA
    49.878000 -97.235520
  • Libraries Annex (not open to the public; please see web page for details)Borrow it
    25 Chancellors Circle (in the Elizabeth Dafoe Library), Winnipeg, MB, R3T 2N2, CA
    49.809961 -97.131878
  • Neil John Maclean Health Sciences LibraryBorrow it
    727 McDermot Avenue (Brodie Centre), 200 Level, Winnipeg, MB, R3E 3P5, CA
    49.903563 -97.160554
  • Sciences and Technology LibraryBorrow it
    186 Dysart Road, Winnipeg, MB, R3T 2M8, CA
    49.811526 -97.133257
  • Seven Oaks General Hospital LibraryBorrow it
    2300 McPhillips Street, Winnipeg, MB, R2V 3M3, CA
    49.955177 -97.148865
  • Sister St. Odilon Library (Misericordia Health Centre)Borrow it
    99 Cornish Avenue, Winnipeg, MB, R3C 1A2, CA
    49.879592 -97.160425
  • St. John's College LibraryBorrow it
    92 Dysart Road, Winnipeg, MB, R3T 2M5, CA
    49.811242 -97.137156
  • Victoria General Hospital LibraryBorrow it
    2340 Pembina Highway, Winnipeg, MB, R3T 2E8, CA
    49.806755 -97.152739
  • William R Newman Library (Agriculture)Borrow it
    66 Dafoe Road, Winnipeg, MB, R3T 2R3, CA
    49.806936 -97.135525
Processing Feedback ...