The Resource Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols, by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger, (electronic resource)

Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols, by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger, (electronic resource)

Label
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Title
Low-Cost Methods for Molecular Characterization of Mutant Plants
Title remainder
Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Statement of responsibility
by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger
Creator
Contributor
Author
Author
Subject
Language
  • eng
  • eng
Summary
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits
http://library.link/vocab/creatorName
Till, Bradley J
Dewey number
  • 631.52
  • 660.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsaut
  • 7Er1M_PjFvk
  • 4p8xsp_ZgOE
  • 4wMu4fLGnf4
  • 2MjTxXCAgMk
  • XjnuVmg1PPs
  • LUmF_BpcHXk
Image bit depth
0
Language note
English
LC call number
  • SB123-123.5
  • S494.5.B563
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Jankowicz-Cieslak, Joanna.
  • Huynh, Owen A.
  • Beshir, Mayada M.
  • Laport, Robert G.
  • Hofinger, Bernhard J.
http://library.link/vocab/subjectName
  • Plant breeding
  • Cytology
  • Nucleic acids
  • Plant Breeding/Biotechnology
  • Biological Techniques
  • Nucleic Acid Chemistry
Label
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols, by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
Dimensions
unknown
Edition
1st ed. 2015.
Extent
1 online resource (X, 35 p. 9 illus., 3 illus. in color.)
File format
multiple file formats
Form of item
online
Governing access note
Open Access
Isbn
9783319162591
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-3-319-16259-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000415186
  • (SSID)ssj0001500656
  • (PQKBManifestationID)11967966
  • (PQKBTitleCode)TC0001500656
  • (PQKBWorkID)11535914
  • (PQKB)10488240
  • (DE-He213)978-3-319-16259-1
  • (EXLCZ)993710000000415186
Label
Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols, by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions.
Dimensions
unknown
Edition
1st ed. 2015.
Extent
1 online resource (X, 35 p. 9 illus., 3 illus. in color.)
File format
multiple file formats
Form of item
online
Governing access note
Open Access
Isbn
9783319162591
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-3-319-16259-1
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000415186
  • (SSID)ssj0001500656
  • (PQKBManifestationID)11967966
  • (PQKBTitleCode)TC0001500656
  • (PQKBWorkID)11535914
  • (PQKB)10488240
  • (DE-He213)978-3-319-16259-1
  • (EXLCZ)993710000000415186

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