The Resource Methods in Microbiology : Yeast Gene Analysis, Second Edition, (electronic resource)

Methods in Microbiology : Yeast Gene Analysis, Second Edition, (electronic resource)

Label
Methods in Microbiology : Yeast Gene Analysis, Second Edition
Title
Methods in Microbiology
Title remainder
Yeast Gene Analysis, Second Edition
Title variation
  • Methods in Microbiology, 36
  • Methods in Microbiology
  • Methods in Microbiology, Volume 36C
  • Yeast Gene Analysis Second Edition, Volume 36C
Creator
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using
Member of
Is Subseries of
Cataloging source
AU-PeEL
http://library.link/vocab/creatorName
Stansfield, Ian
Dewey number
  • 576.028
  • 579.562
Language note
English
LC call number
QR65.N59 2007eb
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Stark, Mike
Series statement
Methods in microbiology
http://library.link/vocab/subjectName
  • Microbiology
  • Mycorrhizas
  • Saccharomyces cerevisiae
  • Yeast fungi
  • Genetic Techniques
  • Yeasts
  • Saccharomyces cerevisiae
  • Fungi
  • Saccharomyces
  • Investigative Techniques
  • Eukaryota
  • Analytical, Diagnostic and Therapeutic Techniques and Equipment
  • Saccharomycetales
  • Organisms
  • Ascomycota
  • Microbiology & Immunology
  • Biology
  • Health & Biological Sciences
Label
Methods in Microbiology : Yeast Gene Analysis, Second Edition, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Abbreviated title
YEAST GENE ANALYSIS
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front cover; Methods in Microbiology; Copyright page; Contents; Series Advisors; Contributors; Preface; Chapter 1. Introduction to Functional Analysis in Yeast; I. The Genome Sequence and how it Changed the World of Yeast; II. Yeast Genetics - New Perspectives; III. Transcriptomics and an Emerging RNA World; IV. Proteomics; V. Metabolomics; VI. The Minimal Genome and the E-Yeast; Acknowledgements; References; Chapter 2. Yeast Genetics and Strain Construction; I. Introduction; II. Growing Yeast; III. Planning Strain Construction; IV. Yeast Mating; V. Sporulating Yeast
  • VI. Tetrad Dissection and Phenotype AnalysisVII. Modifying Strains by Plasmid Integration; VIII. Concluding Remarks; References; Chapter 3. Yeast Transformation; I. Introduction; II. Reagents and Solutions; III. Rapid Transformation; IV. High Efficiency Transformation; V. Microtitre Plate Transformation; VI. Preparation of Transformation-Competent Frozen Yeast Cells; VII. Summary; References; Chapter 4. A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting); I. Introduction; II. Application of PCR Targeting for Functional Studies
  • III. Available Cassettes for PCR TargetingIV. Primers for use with PCR Targeting; V. Application of PCR Targeting for Genome-Wide Studies; VI. Critical Methods; VII. Concluding Remarks; References; Chapter 5. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles; I. Introduction; II. Using Regulated Promoters to Study Essential Genes; III. Heat-Inducible Degron Fusions; IV. Generating Conditional Alleles; References; Chapter 6. Yeast Hybrid Approaches; I. Introduction: The Origins and Composition of the Yeast Two-Hybrid (Y2H) System
  • II. Selecting a System for Y2H AssaysIII. How to use the ''Classic'' Y2H; IV. Specialized Approaches; V. Conclusion; References; Chapter 7. Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions; I. Introduction; II. Steps Involved in an Array-Based Yeast Two-Hybrid Screen; III. Screening for Protein Interactions Using a Yeast Colony Array; IV. Conclusion; Acknowledgments; References; Chapter 8. Reporter Genes and Their Uses in Studying Yeast Gene Expression; I. Introduction to Reporter Genes; II. Generating Reporter Gene Constructs
  • III. Reporter Genes Available for Work in YeastIV. Problems and Pitfalls; V. Conclusions; Acknowledgements; References; Chapter 9. Transcript Analysis: A Microarray Approach; I. Introduction; II. Performing the Microarray Experiment; III. Experimental Design; IV. Computational Analysis; V. Summary and Future Perspectives; Acknowledgements; References; Chapter 10. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts; I. Introduction; II. Microscopic Techniques; III. GFP-Tagging Schemes; IV. Representative GFP-Based Assays; Acknowledgement; References
  • Chapter 11. Immunological Methods
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (731 p.)
Form of item
electronic
Isbn
9786611020439
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000364143
  • (EBL)298270
  • (OCoLC)476073171
  • (SSID)ssj0000275762
  • (PQKBManifestationID)11218683
  • (PQKBTitleCode)TC0000275762
  • (PQKBWorkID)10223043
  • (PQKB)10144585
  • (EXLCZ)991000000000364143
Label
Methods in Microbiology : Yeast Gene Analysis, Second Edition, (electronic resource)
Publication
Note
Description based upon print version of record
Abbreviated title
YEAST GENE ANALYSIS
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front cover; Methods in Microbiology; Copyright page; Contents; Series Advisors; Contributors; Preface; Chapter 1. Introduction to Functional Analysis in Yeast; I. The Genome Sequence and how it Changed the World of Yeast; II. Yeast Genetics - New Perspectives; III. Transcriptomics and an Emerging RNA World; IV. Proteomics; V. Metabolomics; VI. The Minimal Genome and the E-Yeast; Acknowledgements; References; Chapter 2. Yeast Genetics and Strain Construction; I. Introduction; II. Growing Yeast; III. Planning Strain Construction; IV. Yeast Mating; V. Sporulating Yeast
  • VI. Tetrad Dissection and Phenotype AnalysisVII. Modifying Strains by Plasmid Integration; VIII. Concluding Remarks; References; Chapter 3. Yeast Transformation; I. Introduction; II. Reagents and Solutions; III. Rapid Transformation; IV. High Efficiency Transformation; V. Microtitre Plate Transformation; VI. Preparation of Transformation-Competent Frozen Yeast Cells; VII. Summary; References; Chapter 4. A Guided Tour to PCR-based Genomic Manipulations of S. cerevisiae (PCR-targeting); I. Introduction; II. Application of PCR Targeting for Functional Studies
  • III. Available Cassettes for PCR TargetingIV. Primers for use with PCR Targeting; V. Application of PCR Targeting for Genome-Wide Studies; VI. Critical Methods; VII. Concluding Remarks; References; Chapter 5. Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles; I. Introduction; II. Using Regulated Promoters to Study Essential Genes; III. Heat-Inducible Degron Fusions; IV. Generating Conditional Alleles; References; Chapter 6. Yeast Hybrid Approaches; I. Introduction: The Origins and Composition of the Yeast Two-Hybrid (Y2H) System
  • II. Selecting a System for Y2H AssaysIII. How to use the ''Classic'' Y2H; IV. Specialized Approaches; V. Conclusion; References; Chapter 7. Array-Based Yeast Two-Hybrid Screening for Protein-Protein Interactions; I. Introduction; II. Steps Involved in an Array-Based Yeast Two-Hybrid Screen; III. Screening for Protein Interactions Using a Yeast Colony Array; IV. Conclusion; Acknowledgments; References; Chapter 8. Reporter Genes and Their Uses in Studying Yeast Gene Expression; I. Introduction to Reporter Genes; II. Generating Reporter Gene Constructs
  • III. Reporter Genes Available for Work in YeastIV. Problems and Pitfalls; V. Conclusions; Acknowledgements; References; Chapter 9. Transcript Analysis: A Microarray Approach; I. Introduction; II. Performing the Microarray Experiment; III. Experimental Design; IV. Computational Analysis; V. Summary and Future Perspectives; Acknowledgements; References; Chapter 10. GFP-based Microscopic Approaches for Whole Chromosome Analysis in Yeasts; I. Introduction; II. Microscopic Techniques; III. GFP-Tagging Schemes; IV. Representative GFP-Based Assays; Acknowledgement; References
  • Chapter 11. Immunological Methods
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (731 p.)
Form of item
electronic
Isbn
9786611020439
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000364143
  • (EBL)298270
  • (OCoLC)476073171
  • (SSID)ssj0000275762
  • (PQKBManifestationID)11218683
  • (PQKBTitleCode)TC0000275762
  • (PQKBWorkID)10223043
  • (PQKB)10144585
  • (EXLCZ)991000000000364143

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