The Resource Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part A, (electronic resource)

Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part A, (electronic resource)

Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part A
Title
Methods in enzymology
Title remainder
Guide to Yeast Genetics and Molecular Biology : Part A
Title variation
  • Methods in enzymology
  • Methods in Enzymology, Volume 194C
  • Guide to Yeast Genetics and Molecular Biology , Volume 194C
Creator
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study ""Your Favorite Gene"" in yeast.* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* Hi
Is Subseries of
Cataloging source
AU-PeEL
http://library.link/vocab/creatorName
Abelson, John N
Dewey number
  • 572.7
  • 579.562135
Language note
English
LC call number
QP601 .M49
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Simon, Melvin I
  • Guthrie, Christine
  • Fink, Gerald R
http://library.link/vocab/subjectName
  • Molecular Biology
  • Yeast fungi -- Cytology
  • Yeast fungi -- Genetics
  • Yeast fungi -- Molecular aspects
  • Yeasts -- cytology
  • Yeasts -- genetics
  • Biochemistry
  • Genetics
  • Fungi
  • Investigative Techniques
  • Eukaryota
  • Biology
  • Analytical, Diagnostic and Therapeutic Techniques and Equipment
  • Chemistry
  • Biological Science Disciplines
  • Natural Science Disciplines
  • Organisms
  • Disciplines and Occupations
  • Molecular Biology
  • Genetic Techniques
  • Yeasts
Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part A, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Methods in Enzymology; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Section 1: Functional Genomics; Chapter 1: Analysis of Gene Function Using DNA Microarrays; 1. Introduction and Experimental Design; 1.1. Single-mutant analysis; 1.2. Double-mutant analysis; 2. Methods; 2.1. Experimental design; 2.2. Cell growth; 2.3. Total RNA isolation and purification; 2.4. Purification of poly-A RNA; 2.5. Reverse transcription and dye labeling; 2.6. Hybridization; 2.7. Microarray washing; 2.8. Array scanning; 2.9. Gridding and normalization; References
  • Chapter 2: An Introduction to Microarray Data Analysis and Visualization1. Introduction; 1.1. Overview; 1.2. Commonly used terms in microarray data analysis; 1.3. A simple case study; 2. Experimental Design: Single-Sample Versus Competitive Hybridization; 2.1. Single-sample hybridization; 2.2. Competitive hybridization; 2.3. Choosing the best approach; 3. Image Analysis; 3.1. What is a digital image?; 3.2. Data files; 3.3. Software tools; 4. Preprocessing; 4.1. Software tools; 4.2. Calculating ratio values; 4.3. Normalizing ratio values
  • 4.4. Quality assessment, filtering, and handling replicates4.5. Preprocessing Affymetrix arrays; 5. Visualizing Data Using Cluster Analysis; 5.1. Hierarchical clustering; 5.2. Partitioning and network-based approaches; 6. Assessing the Statistical Evidence for Differential Expression; 6.1. Significance analysis of microarrays; 6.2. Limma; 7. Exploring Gene Sets; 7.1. Gene Ontology term mapping; 7.2. Motif searching; 7.3. Network visualization; 7.4. Graphing array data on genome tracks; 8. Managing Data; 8.1. Data persistence and integrity; 8.2. Public data repositories and MIAME compliance
  • AcknowledgmentsReferences; Chapter 3: Genome-Wide Approaches to Monitor Pre-mRNA Splicing; 1. Introduction; 2. Microarray Design; 3. Sample Preparation; 3.1. Cell collection; 3.2. RNA isolation; 3.3. cDNA synthesis; 3.4. Fluorescent labeling of cDNA; 3.5. Microarray hybridization; 3.6. Microarray washing; 4. Microarray Data Collection; 5. Microarray Data Analysis; 5.1. Data normalization; 5.2. Replication; 5.3. Splicing specific data; 5.4. Extracting biological meaning; 6. Future Methodologies; Acknowledgments; References
  • Chapter 4: ChIP-Seq: Using High-Throughput DNA Sequencing for Genome-Wide Identification of Transcription Factor Binding Site1. Introduction; 2. Protocols; 2.1. Chromatin immunoprecipitation; 2.2. Input DNA preparation; 2.3. Illumina sequencing DNA library generation; 2.4. Barcode design and adapter annealing; 2.5. Illumina sequencing; 3. Sequencing Data Management; 4. Genome Analysis Pipeline; 5. Examining Data Quality and Parsing Barcoded Data; 6. Visualization in Genome Browser; 6.1. Low-level analysis; 6.2. High-level analysis; 6.3. Troubleshooting; 7. Conclusion and Future Directions
  • Acknowledgments
Dimensions
unknown
Extent
1 online resource (961 p.)
Form of item
electronic
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)2500000000003084
  • (EBL)579221
  • (OCoLC)813229115
  • (SSID)ssj0000492547
  • (PQKBManifestationID)12214496
  • (PQKBTitleCode)TC0000492547
  • (PQKBWorkID)10479246
  • (PQKB)10198844
  • (EXLCZ)992500000000003084
Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part A, (electronic resource)
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Methods in Enzymology; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Section 1: Functional Genomics; Chapter 1: Analysis of Gene Function Using DNA Microarrays; 1. Introduction and Experimental Design; 1.1. Single-mutant analysis; 1.2. Double-mutant analysis; 2. Methods; 2.1. Experimental design; 2.2. Cell growth; 2.3. Total RNA isolation and purification; 2.4. Purification of poly-A RNA; 2.5. Reverse transcription and dye labeling; 2.6. Hybridization; 2.7. Microarray washing; 2.8. Array scanning; 2.9. Gridding and normalization; References
  • Chapter 2: An Introduction to Microarray Data Analysis and Visualization1. Introduction; 1.1. Overview; 1.2. Commonly used terms in microarray data analysis; 1.3. A simple case study; 2. Experimental Design: Single-Sample Versus Competitive Hybridization; 2.1. Single-sample hybridization; 2.2. Competitive hybridization; 2.3. Choosing the best approach; 3. Image Analysis; 3.1. What is a digital image?; 3.2. Data files; 3.3. Software tools; 4. Preprocessing; 4.1. Software tools; 4.2. Calculating ratio values; 4.3. Normalizing ratio values
  • 4.4. Quality assessment, filtering, and handling replicates4.5. Preprocessing Affymetrix arrays; 5. Visualizing Data Using Cluster Analysis; 5.1. Hierarchical clustering; 5.2. Partitioning and network-based approaches; 6. Assessing the Statistical Evidence for Differential Expression; 6.1. Significance analysis of microarrays; 6.2. Limma; 7. Exploring Gene Sets; 7.1. Gene Ontology term mapping; 7.2. Motif searching; 7.3. Network visualization; 7.4. Graphing array data on genome tracks; 8. Managing Data; 8.1. Data persistence and integrity; 8.2. Public data repositories and MIAME compliance
  • AcknowledgmentsReferences; Chapter 3: Genome-Wide Approaches to Monitor Pre-mRNA Splicing; 1. Introduction; 2. Microarray Design; 3. Sample Preparation; 3.1. Cell collection; 3.2. RNA isolation; 3.3. cDNA synthesis; 3.4. Fluorescent labeling of cDNA; 3.5. Microarray hybridization; 3.6. Microarray washing; 4. Microarray Data Collection; 5. Microarray Data Analysis; 5.1. Data normalization; 5.2. Replication; 5.3. Splicing specific data; 5.4. Extracting biological meaning; 6. Future Methodologies; Acknowledgments; References
  • Chapter 4: ChIP-Seq: Using High-Throughput DNA Sequencing for Genome-Wide Identification of Transcription Factor Binding Site1. Introduction; 2. Protocols; 2.1. Chromatin immunoprecipitation; 2.2. Input DNA preparation; 2.3. Illumina sequencing DNA library generation; 2.4. Barcode design and adapter annealing; 2.5. Illumina sequencing; 3. Sequencing Data Management; 4. Genome Analysis Pipeline; 5. Examining Data Quality and Parsing Barcoded Data; 6. Visualization in Genome Browser; 6.1. Low-level analysis; 6.2. High-level analysis; 6.3. Troubleshooting; 7. Conclusion and Future Directions
  • Acknowledgments
Dimensions
unknown
Extent
1 online resource (961 p.)
Form of item
electronic
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)2500000000003084
  • (EBL)579221
  • (OCoLC)813229115
  • (SSID)ssj0000492547
  • (PQKBManifestationID)12214496
  • (PQKBTitleCode)TC0000492547
  • (PQKBWorkID)10479246
  • (PQKB)10198844
  • (EXLCZ)992500000000003084

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