The Resource Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part B, (electronic resource)

Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part B, (electronic resource)

Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part B
Title
Methods in enzymology
Title remainder
Guide to Yeast Genetics and Molecular Biology : Part B
Title variation
  • Methods in Enzymology, Volume 350C
  • Methods in enzymology
  • Guide to Yeast Genetics and Molecular and Cell Biology - Part B, Volume 350C
Creator
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics
Is Subseries of
Cataloging source
AU-PeEL
http://library.link/vocab/creatorName
Guthrie, Christine
Dewey number
  • 571.29562
  • 579.562
Language note
English
LC call number
QP601 .M49 v.350
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Fink, Gerald R
http://library.link/vocab/subjectName
  • Molecular biology
  • Saccharomyces cerevisiae
  • Yeast
  • Yeast fungi
  • Biochemistry
  • Fungi
  • Investigative Techniques
  • Genetics
  • Biology
  • Biological Science Disciplines
  • Analytical, Diagnostic and Therapeutic Techniques and Equipment
  • Chemistry
  • Eukaryota
  • Natural Science Disciplines
  • Organisms
  • Disciplines and Occupations
  • Genetic Techniques
  • Molecular Biology
  • Yeasts
Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part B, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Abbreviated title
GUIDE TO YEAST GENETICS AND MOLECULAR AND CELL BIOLOGY
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Guide to Yeast Genetics and Molecular and Cell Biology; Copyright Page; Table of Contents; Contributors to Volume 350; Preface; Volume in Series; Section I: Basic Techniques; Chapter 1. Getting Started with Yeast; Chapter 2. How to Set up a Yeast Laboratory; Chapter 3. Constructing Yeast Libraries; Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method; Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria; Chapter 6. LacZ Assays in Yeast; Chapter 7. Analysis of Budding Patterns
  • Chapter 8. Uses and Abuses of HO EndonucleaseChapter 9. Assays for Gene Silencing in Yeast; Section II: Making Mutants; Chapter 10. Classical Mutagenesis Techniques; Chapter 11. Introduction of Point Mutations into Cloned Genes; Chapter 12. Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast; Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro; Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae
  • Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCRChapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods; Chapter 17. Gene Disruption; Chapter 18. Synthetic Dosage Lethality; Section III: Genomics; Chapter 19. Saccharomyces Genome Database; Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD); Chapter 21. Database Resources Relevant to Yeast Biology; Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site
  • Chapter 23. Yeast Genomic Expression Studies Using DNA MicroarraysChapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression; Chapter 25. PCR-Based Engineering of Yeast Genome; Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites; Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome; Section IV: Proteomics; Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein-Nucleic Acid Interactions
  • Chapter 29. Building Protein-Protein Networks by Two- Hybrid Mating StrategyChapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era; Chapter 31. Biochemical Genomics Approach to Map Activities to Genes; Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics; Author Index; Subject Index
Dimensions
unknown
Extent
1 online resource (663 p.)
Form of item
electronic
Isbn
9780080496986
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000364494
  • (EBL)296931
  • (OCoLC)808616204
  • (SSID)ssj0000166503
  • (PQKBManifestationID)11155378
  • (PQKBTitleCode)TC0000166503
  • (PQKBWorkID)10161242
  • (PQKB)11353902
  • (EXLCZ)991000000000364494
Label
Methods in enzymology : Guide to Yeast Genetics and Molecular Biology : Part B, (electronic resource)
Publication
Note
Description based upon print version of record
Abbreviated title
GUIDE TO YEAST GENETICS AND MOLECULAR AND CELL BIOLOGY
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Guide to Yeast Genetics and Molecular and Cell Biology; Copyright Page; Table of Contents; Contributors to Volume 350; Preface; Volume in Series; Section I: Basic Techniques; Chapter 1. Getting Started with Yeast; Chapter 2. How to Set up a Yeast Laboratory; Chapter 3. Constructing Yeast Libraries; Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method; Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria; Chapter 6. LacZ Assays in Yeast; Chapter 7. Analysis of Budding Patterns
  • Chapter 8. Uses and Abuses of HO EndonucleaseChapter 9. Assays for Gene Silencing in Yeast; Section II: Making Mutants; Chapter 10. Classical Mutagenesis Techniques; Chapter 11. Introduction of Point Mutations into Cloned Genes; Chapter 12. Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast; Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro; Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae
  • Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCRChapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods; Chapter 17. Gene Disruption; Chapter 18. Synthetic Dosage Lethality; Section III: Genomics; Chapter 19. Saccharomyces Genome Database; Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD); Chapter 21. Database Resources Relevant to Yeast Biology; Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site
  • Chapter 23. Yeast Genomic Expression Studies Using DNA MicroarraysChapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression; Chapter 25. PCR-Based Engineering of Yeast Genome; Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites; Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome; Section IV: Proteomics; Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein-Nucleic Acid Interactions
  • Chapter 29. Building Protein-Protein Networks by Two- Hybrid Mating StrategyChapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era; Chapter 31. Biochemical Genomics Approach to Map Activities to Genes; Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics; Author Index; Subject Index
Dimensions
unknown
Extent
1 online resource (663 p.)
Form of item
electronic
Isbn
9780080496986
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000364494
  • (EBL)296931
  • (OCoLC)808616204
  • (SSID)ssj0000166503
  • (PQKBManifestationID)11155378
  • (PQKBTitleCode)TC0000166503
  • (PQKBWorkID)10161242
  • (PQKB)11353902
  • (EXLCZ)991000000000364494

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