The Resource Mouse Cell Culture : Methods and Protocols, edited by Andrew Ward, David Tosh, (electronic resource)

Mouse Cell Culture : Methods and Protocols, edited by Andrew Ward, David Tosh, (electronic resource)

Label
Mouse Cell Culture : Methods and Protocols
Title
Mouse Cell Culture
Title remainder
Methods and Protocols
Statement of responsibility
edited by Andrew Ward, David Tosh
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders
Member of
Dewey number
571.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • Q6OtNRzGhq0
  • 4KjxFFrKz-I
Image bit depth
0
Language note
English
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Ward, Andrew.
  • Tosh, David.
Series statement
Methods in Molecular Biology,
Series volume
633
http://library.link/vocab/subjectName
  • Cytology
  • Biochemistry
  • Cell culture
  • Laboratory animals
  • Cell Biology
  • Biochemistry, general
  • Cell Culture
  • Animal Models
Label
Mouse Cell Culture : Methods and Protocols, edited by Andrew Ward, David Tosh, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies -- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures -- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells -- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference -- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation -- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine -- Isolation and Culture of Embryonic Pancreas and Liver -- Isolation and Culture of Mouse Satellite Cells -- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures -- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes -- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells -- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes -- Isolation and Culture of Adult Mouse Hepatocytes -- Isolation and Culture of Mouse Intestinal Cells -- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse -- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry -- Cell Culture of Primary Cerebellar Granule Cells -- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (XI, 250 p. 48 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597450195
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-019-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000010418
  • (SSID)ssj0000327919
  • (PQKBManifestationID)11232981
  • (PQKBTitleCode)TC0000327919
  • (PQKBWorkID)10303569
  • (PQKB)10025446
  • (DE-He213)978-1-59745-019-5
  • (EXLCZ)992670000000010418
Label
Mouse Cell Culture : Methods and Protocols, edited by Andrew Ward, David Tosh, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies -- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures -- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells -- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference -- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation -- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine -- Isolation and Culture of Embryonic Pancreas and Liver -- Isolation and Culture of Mouse Satellite Cells -- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures -- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes -- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells -- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes -- Isolation and Culture of Adult Mouse Hepatocytes -- Isolation and Culture of Mouse Intestinal Cells -- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse -- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry -- Cell Culture of Primary Cerebellar Granule Cells -- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (XI, 250 p. 48 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781597450195
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-019-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000010418
  • (SSID)ssj0000327919
  • (PQKBManifestationID)11232981
  • (PQKBTitleCode)TC0000327919
  • (PQKBWorkID)10303569
  • (PQKB)10025446
  • (DE-He213)978-1-59745-019-5
  • (EXLCZ)992670000000010418

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