The Resource PCR Protocols, edited by Daniel J. Park, (electronic resource)

PCR Protocols, edited by Daniel J. Park, (electronic resource)

Label
PCR Protocols
Title
PCR Protocols
Statement of responsibility
edited by Daniel J. Park
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Protocols, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Protocols, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds
Member of
Is Subseries of
Dewey number
  • 611.01816
  • 599.935
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
NcOXRxLfeDE
Image bit depth
0
Language note
English
LC call number
  • RB155-155.8
  • QH431
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Park, Daniel J.
Series statement
Methods in Molecular Biology,
Series volume
687
http://library.link/vocab/subjectName
  • Human genetics
  • Nucleic acids
  • Medical laboratories
  • Human Genetics
  • Nucleic Acid Chemistry
  • Laboratory Medicine
Label
PCR Protocols, edited by Daniel J. Park, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Setup of a PCR Laboratory -- Long Range PCR with a DNA Polymerase Fusion -- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures -- Lariat-Dependent Nested PCR for Flanking Sequence Determination -- CODEHOP PCR and CODEHOP PCR Primer Design -- Sequencing of Difficult DNA Regions by SAM Sequencing -- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis -- Quantitation of MicroRNAs by Real-Time RT-qPCR -- High-Throughput SuperSAGE -- Deep Cap Analysis of Gene Expression (Deep CAGE) -- Linking Emulsion PCR Haplotype Analysis -- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure -- The Many Faces of MLPA -- Assessing Gene-Specific Methylation Using HRM-Based Analysis -- Alu PCR -- Asynchronous PCR -- Novel Applications of PCR through the Use of DNA Substrates -- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support -- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR -- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis -- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries -- GLOBE: Analysis of DNA-Protein Interaction Analysis -- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues -- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution
Dimensions
unknown
Edition
3.
Extent
1 online resource (XI, 348 p.)
File format
multiple file formats
Form of item
online
Isbn
9781607619444
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-60761-944-4
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000057451
  • (SSID)ssj0000482024
  • (PQKBManifestationID)11344000
  • (PQKBTitleCode)TC0000482024
  • (PQKBWorkID)10524903
  • (PQKB)10781796
  • (DE-He213)978-1-60761-944-4
  • (EXLCZ)992670000000057451
Label
PCR Protocols, edited by Daniel J. Park, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Setup of a PCR Laboratory -- Long Range PCR with a DNA Polymerase Fusion -- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures -- Lariat-Dependent Nested PCR for Flanking Sequence Determination -- CODEHOP PCR and CODEHOP PCR Primer Design -- Sequencing of Difficult DNA Regions by SAM Sequencing -- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis -- Quantitation of MicroRNAs by Real-Time RT-qPCR -- High-Throughput SuperSAGE -- Deep Cap Analysis of Gene Expression (Deep CAGE) -- Linking Emulsion PCR Haplotype Analysis -- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure -- The Many Faces of MLPA -- Assessing Gene-Specific Methylation Using HRM-Based Analysis -- Alu PCR -- Asynchronous PCR -- Novel Applications of PCR through the Use of DNA Substrates -- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support -- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR -- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis -- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries -- GLOBE: Analysis of DNA-Protein Interaction Analysis -- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues -- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution
Dimensions
unknown
Edition
3.
Extent
1 online resource (XI, 348 p.)
File format
multiple file formats
Form of item
online
Isbn
9781607619444
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-60761-944-4
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000057451
  • (SSID)ssj0000482024
  • (PQKBManifestationID)11344000
  • (PQKBTitleCode)TC0000482024
  • (PQKBWorkID)10524903
  • (PQKB)10781796
  • (DE-He213)978-1-60761-944-4
  • (EXLCZ)992670000000057451

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