Coverart for item
The Resource Phage display of peptides and proteins : a laboratory manual, edited by Brian K. Kay, Jill Winter, John McCafferty, (electronic resource)

Phage display of peptides and proteins : a laboratory manual, edited by Brian K. Kay, Jill Winter, John McCafferty, (electronic resource)

Label
Phage display of peptides and proteins : a laboratory manual
Title
Phage display of peptides and proteins
Title remainder
a laboratory manual
Statement of responsibility
edited by Brian K. Kay, Jill Winter, John McCafferty
Creator
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins.Key Features* Prepared by experts in the field* Provides proven laboratory protocols, troubleshooting, and tips* Includes maps, seque
Cataloging source
MiAaPQ
Dewey number
  • 574.19296
  • 576.6482
  • 576/.6482 20
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QR342
LC item number
.P456 1996
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Label
Phage display of peptides and proteins : a laboratory manual, edited by Brian K. Kay, Jill Winter, John McCafferty, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Phage Display of Peptides and Proteins: A Laboratory Manual; Copyright Page; Contents; Contributors; Foreword; Preface; Chapter 1. Biology of the Filamentous Bacteriophage; Introduction to the Life Cycle of the Bacteriophage; The Genome and Its Products; The Ff Bacteriophage Particle; The Infection Process; The Assembly Process; References; Chapter 2. Principles and Applications of Phage Display; Introduction; Phage Display of Natural Peptides; Phage Display of Random Peptides; Phage Display of Protein and Protein Domains; Conclusion; World Wide Web Page; References
  • Chapter 3. Vectors for Phage DisplayPhagemid; Markers; Other Viral Display Vectors; Proteolysis; Valency; Issue of Biological Selection; References; Chapter 4. Microbiological Methods; Protocol 1: Spreading Techniques; Protocol 2: Titering Bacteriophage; Protocol 3: Preparing Phage Particles; Protocol 4: Preparation of Single-Stranded DNA from Viral Particles; Protocol 5: Preparation of Vector RF DNA; References; Chapter 5. Construction of Random Peptide Libraries in Bacteriophage MI3; Introduction; Protocol 1: Assembly of Double-Stranded DNA Insert from Degenerate Oligonucleotides
  • Protocol 2: Preparation of Linearized Vector DNAProtocol 3: Ligation of the Vector and Insert DNA; Protocol 4: Electroporation of Ligated DNA into Bacteria; Protocol 5: Amplification and Harvest of Library Phage; References; Chapter 6. Construction and Screening of Antibody Display Libraries; General Introduction and Background; Production of Antibody Genes and Preparation of Libraries; Protocol 1: Preparation of Lymphocytes as Single-Cell Suspensions from Mouse Spleen Cells; Protocol 2: Preparation of Human Peripheral Blood Lymphocytes
  • Protocol 3: Preparation of Mouse or Human Antibody cDNAConstruction of Antibody V Gene Repertoire; Protocol 4: Preparation and Purification of Primary VH and VL PCR Products; Protocol 5: Preparation and Purification of Linker Fragments; Protocol 6: Assembly of Human Single-Chain Fv Antibody Fragments; Protocol 7: Restriction Digestion of Assembled PCR Fragments; Protocol 8: Preparation of Vector DNA; Protocol 9: Ligation of Insert into Vector; Manipulation of Libraries; Protocol 10: Rescuing Phagemid Libraries; Protocol 11: Selection of Phage-Antibody Libraries by Panning in ""Immunotubes""
  • Protocol 12: Selection of Phage-Antibody Libraries by Biotin SelectionProtocol 13: Introducing Diversity by Chain Shuffling; Screening and Expression of Selected Clones; Protocol 14: Induction of Soluble Antibody Fragments from Phagemid Vectors; Protocol 15: Preparation of Periplasmic Fraction and Purification of Antibodies by Immobilized Metal Affinity Chromatography (IMAC); Protocol 16: Small-Scale Rescue of Phagemid Particles Using Helper Virus; Protocol 17: ELISA Using either Soluble Fragments or Phage Particles; References; Chapter 7. Phagemid-Displayed Peptide Libraries; Introduction
  • Materials
Dimensions
unknown
Extent
1 online resource (369 p.)
Form of item
online
Isbn
9781281033147
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000384335
  • (EBL)317021
  • (OCoLC)476109504
  • (SSID)ssj0000220530
  • (PQKBManifestationID)12075546
  • (PQKBTitleCode)TC0000220530
  • (PQKBWorkID)10143526
  • (PQKB)11456606
  • (MiAaPQ)EBC317021
  • (EXLCZ)991000000000384335
Label
Phage display of peptides and proteins : a laboratory manual, edited by Brian K. Kay, Jill Winter, John McCafferty, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
  • Front Cover; Phage Display of Peptides and Proteins: A Laboratory Manual; Copyright Page; Contents; Contributors; Foreword; Preface; Chapter 1. Biology of the Filamentous Bacteriophage; Introduction to the Life Cycle of the Bacteriophage; The Genome and Its Products; The Ff Bacteriophage Particle; The Infection Process; The Assembly Process; References; Chapter 2. Principles and Applications of Phage Display; Introduction; Phage Display of Natural Peptides; Phage Display of Random Peptides; Phage Display of Protein and Protein Domains; Conclusion; World Wide Web Page; References
  • Chapter 3. Vectors for Phage DisplayPhagemid; Markers; Other Viral Display Vectors; Proteolysis; Valency; Issue of Biological Selection; References; Chapter 4. Microbiological Methods; Protocol 1: Spreading Techniques; Protocol 2: Titering Bacteriophage; Protocol 3: Preparing Phage Particles; Protocol 4: Preparation of Single-Stranded DNA from Viral Particles; Protocol 5: Preparation of Vector RF DNA; References; Chapter 5. Construction of Random Peptide Libraries in Bacteriophage MI3; Introduction; Protocol 1: Assembly of Double-Stranded DNA Insert from Degenerate Oligonucleotides
  • Protocol 2: Preparation of Linearized Vector DNAProtocol 3: Ligation of the Vector and Insert DNA; Protocol 4: Electroporation of Ligated DNA into Bacteria; Protocol 5: Amplification and Harvest of Library Phage; References; Chapter 6. Construction and Screening of Antibody Display Libraries; General Introduction and Background; Production of Antibody Genes and Preparation of Libraries; Protocol 1: Preparation of Lymphocytes as Single-Cell Suspensions from Mouse Spleen Cells; Protocol 2: Preparation of Human Peripheral Blood Lymphocytes
  • Protocol 3: Preparation of Mouse or Human Antibody cDNAConstruction of Antibody V Gene Repertoire; Protocol 4: Preparation and Purification of Primary VH and VL PCR Products; Protocol 5: Preparation and Purification of Linker Fragments; Protocol 6: Assembly of Human Single-Chain Fv Antibody Fragments; Protocol 7: Restriction Digestion of Assembled PCR Fragments; Protocol 8: Preparation of Vector DNA; Protocol 9: Ligation of Insert into Vector; Manipulation of Libraries; Protocol 10: Rescuing Phagemid Libraries; Protocol 11: Selection of Phage-Antibody Libraries by Panning in ""Immunotubes""
  • Protocol 12: Selection of Phage-Antibody Libraries by Biotin SelectionProtocol 13: Introducing Diversity by Chain Shuffling; Screening and Expression of Selected Clones; Protocol 14: Induction of Soluble Antibody Fragments from Phagemid Vectors; Protocol 15: Preparation of Periplasmic Fraction and Purification of Antibodies by Immobilized Metal Affinity Chromatography (IMAC); Protocol 16: Small-Scale Rescue of Phagemid Particles Using Helper Virus; Protocol 17: ELISA Using either Soluble Fragments or Phage Particles; References; Chapter 7. Phagemid-Displayed Peptide Libraries; Introduction
  • Materials
Dimensions
unknown
Extent
1 online resource (369 p.)
Form of item
online
Isbn
9781281033147
Media category
computer
Media type code
c
Specific material designation
remote
System control number
  • (CKB)1000000000384335
  • (EBL)317021
  • (OCoLC)476109504
  • (SSID)ssj0000220530
  • (PQKBManifestationID)12075546
  • (PQKBTitleCode)TC0000220530
  • (PQKBWorkID)10143526
  • (PQKB)11456606
  • (MiAaPQ)EBC317021
  • (EXLCZ)991000000000384335

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