The Resource Plant Epigenetics : Methods and Protocols, edited by Igor Kovalchuk, Franz J. Zemp, (electronic resource)

Plant Epigenetics : Methods and Protocols, edited by Igor Kovalchuk, Franz J. Zemp, (electronic resource)

Label
Plant Epigenetics : Methods and Protocols
Title
Plant Epigenetics
Title remainder
Methods and Protocols
Statement of responsibility
edited by Igor Kovalchuk, Franz J. Zemp
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology
Member of
Dewey number
581.35
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • L5bpfeNGcfA
  • tLKUY7SBwzw
Image bit depth
0
Language note
English
LC call number
QH433
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Kovalchuk, Igor.
  • Zemp, Franz J.
Series statement
Methods in Molecular Biology,
Series volume
631
http://library.link/vocab/subjectName
  • Plant genetics
  • Biochemistry
  • Botany
  • Gene expression
  • Plant Genetics and Genomics
  • Biochemistry, general
  • Plant Sciences
  • Gene Expression
Label
Plant Epigenetics : Methods and Protocols, edited by Igor Kovalchuk, Franz J. Zemp, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Analysis of DNA Methylation in Plants by Bisulfite Sequencing -- Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE -- Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay -- Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis of DNA Methylation in Plants -- Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism -- Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes -- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- qRT-PCR of Small RNAs -- Cloning New Small RNA Sequences -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis -- Metaanalysis of ChIP-chip Data -- Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein–DNA Binding Analyses in Arabidopsis -- cDNA Libraries for Virus-Induced Gene Silencing -- Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay -- Reporter Gene-Based Recombination Lines for Studies of Genome Stability -- Plant Transgenesis
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (X, 273 p. 51 illus., 12 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607616467
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-646-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000010416
  • (SSID)ssj0000391928
  • (PQKBManifestationID)11246165
  • (PQKBTitleCode)TC0000391928
  • (PQKBWorkID)10347761
  • (PQKB)10052656
  • (DE-He213)978-1-60761-646-7
  • (EXLCZ)992670000000010416
Label
Plant Epigenetics : Methods and Protocols, edited by Igor Kovalchuk, Franz J. Zemp, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Analysis of DNA Methylation in Plants by Bisulfite Sequencing -- Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE -- Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay -- Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay -- In Situ Analysis of DNA Methylation in Plants -- Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism -- Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes -- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays -- Northern Blotting Techniques for Small RNAs -- qRT-PCR of Small RNAs -- Cloning New Small RNA Sequences -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods -- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis -- Metaanalysis of ChIP-chip Data -- Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein–DNA Binding Analyses in Arabidopsis -- cDNA Libraries for Virus-Induced Gene Silencing -- Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay -- Reporter Gene-Based Recombination Lines for Studies of Genome Stability -- Plant Transgenesis
Dimensions
unknown
Edition
1st ed. 2010.
Extent
1 online resource (X, 273 p. 51 illus., 12 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607616467
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-646-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000010416
  • (SSID)ssj0000391928
  • (PQKBManifestationID)11246165
  • (PQKBTitleCode)TC0000391928
  • (PQKBWorkID)10347761
  • (PQKB)10052656
  • (DE-He213)978-1-60761-646-7
  • (EXLCZ)992670000000010416

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