The Resource Protocols for Adult Stem Cells, edited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li, (electronic resource)

Protocols for Adult Stem Cells, edited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li, (electronic resource)

Label
Protocols for Adult Stem Cells
Title
Protocols for Adult Stem Cells
Statement of responsibility
edited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In Protocols for Adult Stem Cells, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Critical and cutting edge, Protocols for Adult Stem Cells is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology
Member of
Dewey number
571.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • gvtPiCrK4vU
  • 4j-9MD4uwz8
  • faDOPD8Z9YE
  • c4mwVOtvrGI
Image bit depth
0
Language note
English
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Conboy, Irina M.
  • Schaffer, David V.
  • Barcellos-Hoff, Mary Helen.
  • Li, Song.
Series statement
Methods in Molecular Biology,
Series volume
621
http://library.link/vocab/subjectName
  • Cytology
  • Cell culture
  • Stem cells
  • Cell Biology
  • Cell Culture
  • Stem Cells
Label
Protocols for Adult Stem Cells, edited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells -- Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies -- Use of Stem Cell Markers in Dissociated Mammary Populations -- Isolation of Adult Hippocampal Neural Progenitors -- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors -- In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors -- Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors -- Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix -- Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis -- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy -- Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle
Dimensions
unknown
Edition
1.
Extent
1 online resource (X, 207 p. 20 illus., 1 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607610632
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-063-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2900000000000198
  • (SSID)ssj0000391934
  • (PQKBManifestationID)11308878
  • (PQKBTitleCode)TC0000391934
  • (PQKBWorkID)10347114
  • (PQKB)11094894
  • (DE-He213)978-1-60761-063-2
  • (EXLCZ)992900000000000198
Label
Protocols for Adult Stem Cells, edited by Irina M. Conboy, David V. Schaffer, Mary Helen Barcellos-Hoff, Song Li, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells -- Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies -- Use of Stem Cell Markers in Dissociated Mammary Populations -- Isolation of Adult Hippocampal Neural Progenitors -- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors -- In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors -- Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors -- Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix -- Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis -- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells -- Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy -- Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle
Dimensions
unknown
Edition
1.
Extent
1 online resource (X, 207 p. 20 illus., 1 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607610632
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60761-063-2
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2900000000000198
  • (SSID)ssj0000391934
  • (PQKBManifestationID)11308878
  • (PQKBTitleCode)TC0000391934
  • (PQKBWorkID)10347114
  • (PQKB)11094894
  • (DE-He213)978-1-60761-063-2
  • (EXLCZ)992900000000000198

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